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  • 小鼠抗RPA2單克隆抗體

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    • 品牌 : 通蔚生物
    • 目錄號 : TW11909
    • 應用 : 僅供科研使用
    • 保存條件 : 低溫保存
    • 貨期 : 現貨
    • 商品庫存:20
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中文名稱: 小鼠抗RPA2單克隆抗體    

英文名稱: Anti-RPA2 mouse monoclonal antibody

別    名: replication protein A2; REPA2; RPA32; RP-A p32; RP-A p34

相關類別: 一抗

儲    存: 冷凍(-20℃)

宿    主: Mouse

    原: RPA2

反應種屬: Human

標 記 物: Unconjugate

克隆類型: Mouse Monoclonal

技術規格

Background:

As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.

Applications:

WB, IP, IF

Name of antibody:

RPA2

Immunogen:

Fusion protein of human RPA2

Full name:

replication protein A2

Synonyms:

REPA2; RPA32; RP-A p32; RP-A p34

SwissProt:

P15927

WB Predicted band size:

29 kDa

WB Positive control:

MCF7, Hela and HUVEC cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

Hela cells

IF Recommend dilution:

100-200












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